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Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner

Lookup NU author(s): Professor Anthony Thody


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Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin-E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2-production machinery. It is unclear whether EM-produced PGE 2 contributes to UVR-induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype-1 and -4 donors, followed by assessment of PGE2 production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase-A2, cyclooxygenase-1, cytoplasmic prostaglandin-E synthase and microsomal prostaglandin-E synthase-1, -2. Epidermal melanocytes produced PGE2 under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase-2 (COX-2) mRNA and a selective COX-2 inhibitor (NS-398) reduced PGE2 production. Ultraviolet B-induced PGE2 production was positively correlated with skin phototype-1, despite variability between individual EM donors. By contrast, there was no correlation between PGE2 production by EM and their melanogenic status. Thus, EM may contribute to UVR-induced erythema, with role of donor skin phototype more important than their melanogenic status. © 2010 John Wiley & Sons A/S.

Publication metadata

Author(s): Gledhill K, Rhodes L, Brownrigg M, Haylett A, Masoodi M, Thody A, Nicolaou A, Tobin D

Publication type: Article

Publication status: Published

Journal: Pigment Cell and Melanoma Research

Year: 2010

Volume: 23

Issue: 3

Pages: 394-403

Print publication date: 17/03/2010

ISSN (print): 1755-1471

ISSN (electronic): 1755-148X

Publisher: Wiley-Blackwell


DOI: 10.1111/j.1755-148X.2010.00696.x

PubMed id: 20236442


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Funder referenceFunder name
Centre for Skin Sciences, School of Life Sciences, University of Bradford
WT077714Wellcome Trust