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Isobaric protein and peptide quantification: Perspectives and issues

Lookup NU author(s): Dr Achim Treumann


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An important challenge for proteomics is the ability to compare protein levels across biological samples. Since their introduction, isotopic and isobaric peptide labeling have played an important role in relative quantitative comparisons of proteomes. One important drawback of most of the isotopic-labeling techniques is an increase in sample complexity. This problem was successfully addressed with the construction of isobaric labeling strategies, such as isobaric tag for relative and absolute quantification (iTRAQ), tandem mass tagging, the cleavable isobaric affinity tag, dimethylated leucines and isobaric peptide termini labeling. Furthermore, numerous applications for multiplexing using iTRAQ and tandem mass tagging have been reported. © 2010 Expert Reviews Ltd.

Publication metadata

Author(s): Treumann A, Thiede B

Publication type: Review

Publication status: Published

Journal: Expert Review of Proteomics

Year: 2010

Volume: 7

Issue: 5

Pages: 647-653

Print publication date: 01/10/2010

ISSN (print): 1478-9450

ISSN (electronic): 1744-8387


DOI: 10.1586/epr.10.29