Browse by author
Lookup NU author(s): Dr Olivier Sparagano
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points of bacterial multiplication and the time-spans between the inoculum and the maximum of rickettsial copies were increased in consecutive order from eukaryotic cells, I. ricinus to D. marginatus systems. In dynamic system, multiplication maximum of R. slovaca was achieved 9 days earlier in I. ricinus; however, the number of rickettsial DNA copies was ~3.6×106 more in D. marginatus. © 2010 Blackwell Verlag GmbH.
Author(s): Špitalská E, Sparagano O, Boldiš V
Publication type: Article
Publication status: Published
Journal: Transboundary and Emerging Diseases
Print publication date: 23/04/2010
ISSN (print): 1865-1674
ISSN (electronic): 1865-1682
PubMed id: 20537110
Altmetrics provided by Altmetric