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Phylogenetic and functional conservation of the NKR-P1F and NKR-P1G receptors in rat and mouse

Lookup NU author(s): Professor Colin Brooks

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Abstract

Two clusters of rat Nkrp1 genes can be distinguished based on phylogenetic relationships and functional characteristics. The proximal (centromeric) cluster encodes the well-studied NKR-P1A and NKR-P1B receptors and the distal cluster, the largely uncharacterized, NKR-P1F and NKR-P1G receptors. The inhibitory NKR-P1G receptor is expressed only by the Ly49s3⁺ NK cell subset as detected by RT-PCR, while the activating NKR-P1F receptor is detected in both Ly49s3⁺ and NKR-P1B⁺ NK cells. The mouse NKR-P1G ortholog is expressed by both NKR-P1D⁻ and NKR-P1D⁺ NK cells in C57BL/6 mice. The rat and mouse NKR-P1F and NKR-P1G receptors demonstrate a striking, cross-species conservation of specificity for Clr ligands. NKR-P1F and NKR-P1G reporter cells reacted with overlapping panels of tumour cell lines and with cells transiently transfected with rat Clr2, Clr3, Clr4, Clr6 and Clr7 and mouse Clrc, Clrf, Clrg and Clrd/x, but not with Clr11 or Clrb, which serve as ligands for NKR-P1 from the proximal cluster. These data suggest that the conserved NKR-P1F and NKR-P1G receptors function as promiscuous receptors for a rapidly evolving family of Clr ligands in rodent NK cells.

Publication metadata

Author(s): Kveberg L, Dai KZ, Inngjerdingen M, Brooks CG, Fossum S, Vaage JT

Publication type: Article

Publication status: Published

Journal: Immunogenetics

Year: 2011

Volume: 63

Issue: 7

Pages: 429-436

Print publication date: 01/07/2011

Online publication date: 16/03/2011

Acceptance date: 17/02/2011

Date deposited: 20/10/2014

ISSN (print): 0093-7711

ISSN (electronic): 1432-1211

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00251-011-0520-1

DOI: 10.1007/s00251-011-0520-1

PubMed id: 21409442

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