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A quantitative inverse relationship between connexin32 expression and cell proliferation in a rat hepatoma cell line

Lookup NU author(s): Dr Tao Chen

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Abstract

Gap junctions comprised of connexin proteins are involved in direct intercellular communication and the regulation of cell behaviour and homeostasis. Reduced connexin expression and loss of gap junction function is a characteristic of many cancer cells and of the effect of many non-genotoxic carcinogens that induce cell proliferation. Moreover, when certain cancer cell lines are transfected with specific connexin genes, cells can regain control over proliferation. We have employed RNA interference and dexamethasone to modulate connexin32 expression in MH1C1 cells to a range of concentrations. This allowed the determination of the quantitative relationship between connexin32 protein expression and cell proliferation. The magnitude of cell proliferation, measured by bromodeoxyuridine incorporation, was inversely proportional to the level of connexin32 expression. Q-PCR indicated a lack of change of expression of a range of cell cycle-related genes at 24 h. The inverse relationship between Cx32 expression and proliferation was continuous, and a threshold level of reduction of connexin32 was not observable for an influence on proliferation.

Publication metadata

Author(s): Chen T; Edwards GO; Jondhale S; Chipman JK

Publication type: Article

Publication status: Published

Journal: Toxicology

Year: 2008

Volume: 253

Issue: 1-3

Pages: 46-52

ISSN (print): 0300-483X

ISSN (electronic): 1879-3185

Publisher: Elsevier Ireland Ltd

URL: http://dx.doi.org/10.1016/j.tox.2008.08.010

DOI: 10.1016/j.tox.2008.08.010

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