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Ammonia-oxidizing bacteria in wastewater

Lookup NU author(s): Dr Micol Bellucci, Professor Thomas CurtisORCiD


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Ammonia-oxidizing bacteria (AOB) have a key role in the conversion of ammonia to nitrite in wastewater treatment plants (WWTPs). The characterization of AOB communities in such systems requires the use of genomic methods as AOB are difficult to isolate from environmental samples. Fluorescence in situ hybridization (FISH) using fluorescently labeled probes targeting 16S rRNA molecules provides a robust tool for the detection and quantification of AOB populations in biofilms and activated sludge flocs. The abundance of AOB may be also determined by real-time quantitative polymerase chain reaction (qPCR) using primers that amplify either the 16S rRNA or amoA genes. The evaluation of changes in the AOB community in time and space can be undertaken by PCR amplification of these gene fragments followed by denaturing gradient gel electrophoresis (PCR-DGGE). In this chapter, we summarize the most commonly applied procedures for the analysis of the AOB in wastewater, emphasizing their advantages and limitations.

Publication metadata

Author(s): Bellucci M, Curtis TP

Editor(s): Klotz, M.G., Stein, L.Y.

Publication type: Book Chapter

Publication status: Published

Book Title: Research on Nitrification and Related Processes, Part B

Year: 2011

Volume: 496

Pages: 269-286

Print publication date: 01/01/2011

Edition: 1st

Series Title: Methods in Enzymology

Publisher: Academic Press

Place Published: Maryland Heights, Missouri, United States


DOI: 10.1016/B978-0-12-386489-5.00011-7

Notes: ISSN: 0076-6879 (print) 1557-7988 (online)

Library holdings: Search Newcastle University Library for this item

ISBN: 9780123864895