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Multi-Excitation Fluorescence Spectroscopy for Analysis of Non-Alcoholic Fatty Liver Disease

Lookup NU author(s): Professor Quentin AnsteeORCiD


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Background and Objectives: The increasing incidence of non-alcoholic fatty liver diseases (NAFLD) and the consequent progression to cirrhosis is expected to become a major cause of liver transplantation. This will exacerbate the organ donor shortage and mean that 'marginal' fatty liver grafts are more frequently used. Autofluorescence spectroscopy is a fast, objective, and non-destructive method to detect change in the endogenous fluorophores distribution and could prove to be a valuable tool for NAFLD diagnosis and transplant graft assessment. Materials and Methods: A system was constructed consisting of a fibre probe with two laser diodes that provided excitation light at 375 and 405 nm, and an imaging spectrograph system. This was used to distinguish fluorescence spectra acquired from the harvested livers from mice with NAFLD of differing severity (healthy, mild steatotic and steatohepatitic). The fluorescence data were entered into a sparse multiclass probabilistic algorithm for disease classification. Histopathology, thiobarbituric acid reactive substances (TBARS) and alanine transaminase (ALT) assays were conducted in addition to the fluorescence measurements Results: TBARS and ALT assays enabled differentiation of the steatohepatitic group from the mild steatosis and control groups (P <= 0.028) but failed to separate the mild steatotic group from the control group. The three groups were all clearly differentiated from each other using fluorescence spectroscopy, and classification accuracy was found to be 95%. Conclusion: Fluorescence spectroscopy appears to be a promising approach for the analysis of diseased liver tissue. Lasers Surg. Med. 43:392-400, 2011. (C) 2011 Wiley-Liss, Inc.

Publication metadata

Author(s): Sauvage VR, Levene AP, Nguyen HT, Wood TC, Kudo H, Concas D, Thomas HC, Thursz MR, Goldin RD, Anstee QM, Elson DS

Publication type: Article

Publication status: Published

Journal: Lasers in Surgery and Medicine

Year: 2011

Volume: 43

Issue: 5

Pages: 392-400

Print publication date: 14/06/2011

ISSN (print): 0196-8092

ISSN (electronic): 1096-9101

Publisher: John Wiley & Sons, Inc.


DOI: 10.1002/lsm.21064


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Funder referenceFunder name
Higher Education Funding Council for England (HEFCE)