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Effect of Sample Thickness on the Extracted Near-Infrared Bulk Optical Properties of Bacillus subtilis in Liquid Culture

Lookup NU author(s): Elitsa Dzhongova, Professor Colin Harwood, Dr Suresh Thennadil


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In order to determine the bulk optical properties of a Bacillus subtilis culture during growth phase we investigated the effect of sample thickness on measurements taken with different measurement configurations, namely total diffuse reflectance and total diffuse transmittance. The bulk optical properties were extracted by inverting the measurements using the radiative transfer theory. While the relationship between reflectance and biomass changes with sample thickness and the intensity (absorbance) levels vary significantly for both reflectance and transmittance measurements, the extracted optical properties show consistent behavior in terms of both the relationship with biomass and magnitude. This observation indicates the potential of bulk optical properties for building models that could be more easily transferable compared to those built using raw measurements.

Publication metadata

Author(s): Dzhongova E, Harwood CR, Thennadil SN

Publication type: Article

Publication status: Published

Journal: Applied Spectroscopy

Year: 2011

Volume: 65

Issue: 11

Pages: 1314-1320

Print publication date: 01/11/2011

ISSN (print): 0003-7028

ISSN (electronic): 1943-3530

Publisher: Society of Applied Spectroscopy


DOI: 10.1366/11-06338


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Funder referenceFunder name
Marie Curie FP6 (INTROSPECT)