Toggle Main Menu Toggle Search

Open Access padlockePrints

A Novel Checkpoint and RPA Inhibitory Pathway Regulated by Rif1

Lookup NU author(s): Yuan Xue, Dr Michael Rushton, Dr Laura Maringele

Downloads


Abstract

Cells accumulate single-stranded DNA (ssDNA) when telomere capping, DNA replication, or DNA repair is impeded. This accumulation leads to cell cycle arrest through activating the DNA-damage checkpoints involved in cancer protection. Hence, ssDNA accumulation could be an anti-cancer mechanism. However, ssDNA has to accumulate above a certain threshold to activate checkpoints. What determines this checkpoint-activation threshold is an important, yet unanswered question. Here we identify Rif1 (Rap1-Interacting Factor 1) as a threshold-setter. Following telomere uncapping, we show that budding yeast Rif1 has unprecedented effects for a protein, inhibiting the recruitment of checkpoint proteins and RPA (Replication Protein A) to damaged chromosome regions, without significantly affecting the accumulation of ssDNA at those regions. Using chromatin immuno-precipitation, we provide evidence that Rif1 acts as a molecular "band-aid" for ssDNA lesions, associating with DNA damage independently of Rap1. In consequence, small or incipient lesions are protected from RPA and checkpoint proteins. When longer stretches of ssDNA are generated, they extend beyond the junction-proximal Rif1-protected regions. In consequence, the damage is detected and checkpoint signals are fired, resulting in cell cycle arrest. However, increased Rif1 expression raises the checkpoint-activation threshold to the point it simulates a checkpoint knockout and can also terminate a checkpoint arrest, despite persistent telomere deficiency. Our work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells.


Publication metadata

Author(s): Xue Y, Rushton MD, Maringele L

Publication type: Article

Publication status: Published

Journal: PLoS Genetics

Year: 2011

Volume: 7

Issue: 12

Print publication date: 15/12/2011

Date deposited: 02/03/2012

ISSN (electronic): 1553-7390

Publisher: Public Library of Science

URL: http://dx.doi.org/10.1371/journal.pgen.1002417

DOI: 10.1371/journal.pgen.1002417


Altmetrics

Altmetrics provided by Altmetric


Funding

Funder referenceFunder name
81164Wellcome Trust

Share