Browse by author
Lookup NU author(s): Dr Evan Mulligan,
Dr Natalie Ferry,
Dr Gordon Port,
Professor Angharad MR GatehouseORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
The potential for transgenic oilseed rape (OSR) plants expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) to affect the field slug Deroceras reticulatum was investigated. Characterization of proteolytic digestive enzymes from the slug's digestive gland demonstrated that all age classes of D. reticulatum utilise the OC-1 sensitive cysteine protease, cathepsin-L. Vegetative tissues of transgenic OSR plants were shown to express OC-1 at levels of 0.025-0.1% of total soluble protein. Bio-assays were carried out over a 6 week period to determine the effects of OC-1-expressing OSR on the growth, development and survival of D. reticulatum juveniles; effects on plant damage were also monitored. Despite OC-1 being both a potent inhibitor of digestive proteolysis in vitro and reducing endogenous proteolysis in vivo, slugs in the experimental group performed significantly better in terms of survival and development; furthermore, plant damage was also significantly greater in this group.
Author(s): Mulligan EA, Ferry N, Port G, Gatehouse AMR, Walters K
Editor(s): Dussart, G.B.J.
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: Slugs and Snails: Agricultural, Veterinary and Environmental Perspectives
Year of Conference: 2003
Publisher: British Crop Protection Council
Library holdings: Search Newcastle University Library for this item
Series Title: British Crop Protection Council Symposium Proceedings