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Selective partition of plasmid DNA and RNA from crude Escherichia coli cell lysate by aqueous two-phase systems

Lookup NU author(s): Professor Andy Lyddiatt

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Abstract

In this paper, the partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and dipotassium phosphate aqueous two-phase systems (ATPS) by adding up of NaCl salt was studied with crude Escherichia coli (E. coli) cell lysate. The partition of nucleic acid was studied in an ATPS composed of 17% (w/w) PEG300, 14% (w/w) di-potassium phosphate (designated as 17/14) and 40% (w/w) lysate. It is demonstrated that in a 17/14 system with 40% (w/w) lysate, at least 2% (w/w) or 342 mM (kg ATPS)(-1) NaCl were able to selective partition the pDNA to the bottom phase. (C) 2011 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Luechau F, Ling TC, Lyddiatt A

Publication type: Article

Publication status: Published

Journal: Biochemical Engineering Journal

Year: 2011

Volume: 55

Issue: 3

Pages: 230-232

Print publication date: 05/05/2011

ISSN (print): 1369-703X

ISSN (electronic): 1873-295X

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.bej.2011.04.014

DOI: 10.1016/j.bej.2011.04.014

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