Toggle Main Menu Toggle Search

Open Access padlockePrints

Soj/ParA stalls DNA replication by inhibiting helix formation of the initiator protein DnaA

Lookup NU author(s): Graham Scholefield, Professor Jeff ErringtonORCiD, Professor Heath Murray


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Control of DNA replication initiation is essential for normal cell growth. A unifying characteristic of DNA replication initiator proteins across the kingdoms of life is their distinctive AAA + nucleotide-binding domains. The bacterial initiator DnaA assembles into a right-handed helical oligomer built upon interactions between neighbouring AAA + domains, that in vitro stretches DNA to promote replication origin opening. The Bacillus subtilis protein Soj/ParA has previously been shown to regulate DnaA-dependent DNA replication initiation; however, the mechanism underlying this control was unknown. Here, we report that Soj directly interacts with the AAA + domain of DnaA and specifically regulates DnaA helix assembly. We also provide critical biochemical evidence indicating that DnaA assembles into a helical oligomer in vivo and that the frequency of replication initiation correlates with the extent of DnaA oligomer formation. This work defines a significant new regulatory mechanism for the control of DNA replication initiation in bacteria. The EMBO Journal (2012) 31, 1542-1555. doi:10.1038/emboj.2012.6; Published online 27 January 2012

Publication metadata

Author(s): Scholefield G, Errington J, Murray H

Publication type: Article

Publication status: Published

Journal: EMBO Journal

Year: 2012

Volume: 31

Issue: 6

Pages: 1542-1555

Print publication date: 27/01/2012

ISSN (print): 0261-4189

ISSN (electronic): 1460-2075

Publisher: Nature Publishing Group


DOI: 10.1038/emboj.2012.6


Altmetrics provided by Altmetric


Funder referenceFunder name
Royal Society