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Simultaneous identification of five marine fish pathogens belonging to the genera Tenacibaculum, Vibrio, Photobacterium and Pseudomonas by reverse line blot hybridization

Lookup NU author(s): Narut Thanantong, Dr Olivier Sparagano

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Abstract

The aim of this work was to develop a reverse line blot hybridization (RLB) assay for the identification of five fish pathogens (Tenacibaculum soleae, Tenacibaculum maritimum, Vibrio harveyi, Photobacterium damselae and Pseudomonas baetica) of importance in marine aquaculture. Species-specific probes were designed targeting the 16S-23S intergenic spacer region (ISR) or the 23S rRNA gene. Reference and clinical strains of each target species were all correctly identified by the RLB assay, whereas all non-target strains gave unambiguous negative results with the exception of cross-reactivity between the V. harveyi probe and V. campbellii PCR product. Sensitivity limits of the RLB assay using pure cultures varied among the five target organisms from 1 to 100 pg of genomic DNA. To our knowledge, this is the first RLB protocol developed for identification of bacterial fish pathogens, and also constitutes the first molecular diagnosis tool available for P. baetica. It will be helpful for epidemiological studies and for the control of diseases in aquaculture. (C) 2011 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Lopez JR, Navas JI, Thanantong N, de la Herran R, Sparagano OAE

Publication type: Article

Publication status: Published

Journal: Aquaculture

Year: 2012

Volume: 324

Pages: 33-38

Print publication date: 06/11/2011

ISSN (print): 0044-8486

ISSN (electronic): 1873-5622

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.aquaculture.2011.10.043

DOI: 10.1016/j.aquaculture.2011.10.043

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