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Lookup NU author(s): Professor Michael Goodfellow
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Author(s): Oldfield C, Bonella H, Renwick L, Dodson HI, Alderson G, Goodfellow M
Publication type: Article
Publication status: Published
Journal: Antonie van Leeuwenhoek
Year: 2004
Volume: 85
Issue: 4
Pages: 317-326
ISSN (print): 0003-6072
ISSN (electronic): 1572-9699
Publisher: Springer Netherlands
URL: http://dx.doi.org/10.1023/B:ANTO.0000020383.66622.4d
DOI: 10.1023/B:ANTO.0000020383.66622.4d
Notes: Equine Rhodococcus equi virulence is positively correlated with the expression of a gene, known as vap A, that is located on a circular plasmid. A variant of vap A, known as vap B has been detected in some R. equi isolates from pigs. A better understanding of the biology and epidemiology of R. equi infections is needed, including the distribution of vap A+ and vap B+ strains in animals and the environment. To this end, a polymerase strain reaction technique was developed which permits the selective amplification of vap A and vap B genes in R. equi strains. The technique can be used to accurately designate R. equi isolates as vap A+, vap B+ or vap -. Statistical analysis using the Fisher Exact Test confirmed that the high frequency of vap A+ amongst equine isolates is significant. The experiments were designed by Dr C Oldfield and the corresponding author who also wrote the paper.
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