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Rapid determination of vap A/vap B genotypic in Rhodococcus equi using a differential polymerase chain reaction method

Lookup NU author(s): Professor Michael Goodfellow


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Publication metadata

Author(s): Oldfield C, Bonella H, Renwick L, Dodson HI, Alderson G, Goodfellow M

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2004

Volume: 85

Issue: 4

Pages: 317-326

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer Netherlands


DOI: 10.1023/B:ANTO.0000020383.66622.4d

Notes: Equine Rhodococcus equi virulence is positively correlated with the expression of a gene, known as vap A, that is located on a circular plasmid. A variant of vap A, known as vap B has been detected in some R. equi isolates from pigs. A better understanding of the biology and epidemiology of R. equi infections is needed, including the distribution of vap A+ and vap B+ strains in animals and the environment. To this end, a polymerase strain reaction technique was developed which permits the selective amplification of vap A and vap B genes in R. equi strains. The technique can be used to accurately designate R. equi isolates as vap A+, vap B+ or vap -. Statistical analysis using the Fisher Exact Test confirmed that the high frequency of vap A+ amongst equine isolates is significant. The experiments were designed by Dr C Oldfield and the corresponding author who also wrote the paper.


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