Browse by author
Lookup NU author(s): Professor Colin McGuckin, Dr Nicolas Forraz
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Haemopoietic stem/progenitor cell (HSPC) development is regulated by extrinsic and intrinsic stimuli. Extrinsic modulators include growth factors and cell adhesion molecules, whereas intrinsic regulation is achieved with many transcription factor families, of which the HOX gene products are known to be important in haemopoiesis. Umbilical cord blood CD133+ HSPC proliferation potential was tested in liquid culture with 'TPOFLK' (thrombopoietin, flt-3 ligand and c-kit ligand, promoting HSPC survival and self-renewal), in comparison to 'K36EG' (c-kit-ligand, interleukins-3 and -6, erythropoietin and granulocyte colony-stimulating factor, inducing haemopoietic differentiation). TPOFLK induced a higher CD133+ HSPC proliferation (up to 60-fold more, at week 8) and maintained a higher frequency of the primitive colony-forming cells than K36EG. Quantitative polymerase chain reaction analysis revealed opposite expression patterns for specific HOX genes in expanding cord blood CD133+ HSPC. After 8 weeks in liquid culture, TPOFLK increased the expression of HOX B3, B4 and A9 (associated with uncommitted HSPC) and reduced the expression of HOX B8 and A10 (expressed in committed myeloid cells) when compared to K36EG. These results suggest that TPOFLK induces CD133+ HSPC proliferation, self-renewal and maintenance, up-regulation of HOX B3, B4 and A9 and down-regulation of HOX B8 and A10 gene expression.
Author(s): McGuckin CP, Forraz N, Pettengell R, Thompson A
Publication type: Article
Publication status: Published
Journal: Cell Proliferation
Year: 2004
Volume: 37
Issue: 4
Pages: 295-306
Print publication date: 01/01/2004
ISSN (print): 0960-7722
ISSN (electronic): 1365-2184
Publisher: Wiley-Blackwell Publishing Ltd
URL: http://dx.doi.org/10.1111/j.1365-2184.2004.00313.x
DOI: 10.1111/j.1365-2184.2004.00313.x
Altmetrics provided by Altmetric
