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Expression, crystallization, preliminary X-ray data analysis of NT-ALS9, a fungal adhesin from Candida albicans

Lookup NU author(s): Professor Paula SalgadoORCiD

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Abstract

Candida albicans is a common human fungal commensal that can also cause a range of infections from skin/mucosal `thrush' to severe systemic candidiasis. Adherence to host cells is one of the key determinants of Candida pathogenesis. The Als family of surface proteins has been implicated in adhesion of C. albicans, yet limited information has been published on the structure and mechanism of these fungal adhesins. The N-terminal region of these proteins has been shown to possess adhesive properties, making it a possible target for new therapeutic strategies. Recombinant NT-Als9-2 from C. albicans (residues 18-329) was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.0 Å resolution. The crystals belonged to space group P212121, with unit-cell parameters a = 34.73, b = 68.71, c = 120.03 Å, [alpha] = [beta] = [gamma] = 90° and one molecule in the asymmetric unit. Platinum-derivatized crystals belonged to the same space group, with similar unit-cell parameters, although they were not completely isomorphous.

Publication metadata

Author(s): Salgado PS, Yan R, Rowan F, Cota E

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications

Year: 2011

Volume: 67

Issue: 4

Pages: 467-470

Print publication date: 01/04/2011

ISSN (electronic): 1744-3091

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1107/S1744309111003460

DOI: 10.1107/S1744309111003460

PubMed id: 21505243

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