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Lookup NU author(s): Dr Paula SalgadoORCiD
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The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage ϕ6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629–632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3′ terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
Author(s): Laurila MRL, Salgado PS, Stuart DI, Grimes JM, Bamford DH
Publication type: Article
Publication status: Published
Journal: Journal of General Virology
ISSN (print): 0022-1317
ISSN (electronic): 1465-2099
Publisher: Society for General Microbiology
PubMed id: 15659773
Notes: Also available at: http://vir.sgmjournals.org/content/86/2/521.long
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