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Back-priming mode of Phi6 RNA-dependent RNA polymerase

Lookup NU author(s): Professor Paula SalgadoORCiD


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The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage ϕ6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629–632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3′ terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

Publication metadata

Author(s): Laurila MRL, Salgado PS, Stuart DI, Grimes JM, Bamford DH

Publication type: Article

Publication status: Published

Journal: Journal of General Virology

Year: 2005

Volume: 86

Issue: 2

ISSN (print): 0022-1317

ISSN (electronic): 1465-2099

Publisher: Society for General Microbiology


DOI: 10.1099/vir.0.80492-0

PubMed id: 15659773

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