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Flow cytometric identification of candidate normal stem cell populations in CD45-negative B-cell precursor acute lymphoblastic leukaemia

Lookup NU author(s): Professor Hermann Josef Vormoor

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Abstract

CD45-negative B-cell precursor acute lymphoblastic leukaemia (ALL) provides a unique model to study the stem cell compartment in ALL as leukaemic CD34-positive cells, unlike their normal counterparts, do not express CD45. By increasing the number of events analysed to 10(6), storing only the events in the region of interest (storage gate), using appropriate isotype controls and stringent washing procedures, a flow cytometric protocol was established to characterize rare CD34+ CD19- events. In eight of 12 patients (67%) with CD45-negative B-cell precursor ALL, a distinct CD34+ CD19- CD45+ candidate normal stem cell population could be detected. In one patient analysed by four-colour staining, the CD34+ CD19- CD45+ cells, unlike the CD45-negative leukaemic cells, expressed CD117 (c-kit), providing further evidence that these cells represent residual nonleukaemic normal cells. By multiparameter analysis, this population of candidate normal stem cells could be separated from contaminating leukaemic CD34+ CD19- CD45- cells, which were detected in 11 of the 12 patients within the CD34+ CD19- compartment.

Publication metadata

Author(s): Vormoor J, Baersch G, Baumann M, Ritter J, Jürgens H

Publication type: Article

Publication status: Published

Journal: British Journal of Haematology

Year: 1998

Volume: 100

Issue: 3

Pages: 501-508

Print publication date: 01/03/1998

ISSN (print): 0007-1048

ISSN (electronic): 1365-2141

URL: http://dx.doi.org/10.1046/j.1365-2141.1998.00610.x

DOI: 10.1046/j.1365-2141.1998.00610.x

Notes: 0007-1048 (Print) Journal Article

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