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SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

Lookup NU author(s): Dr Olivier Binda

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Abstract

The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7^me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESCs). H2AZK7^me1 and the repressive H3K27^me3 mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESCs led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESCs require Setd6 for self-renewal and portray H2AZK7^me1 as a marker of cellular differentiation.

Publication metadata

Author(s): Binda O, Sevilla A, LeRoy G, Lemischka IR, Garcia BA, Richard S

Publication type: Article

Publication status: Published

Journal: Epigenetics

Year: 2013

Volume: 8

Issue: 2

Pages: 177-183

Print publication date: 01/02/2013

Date deposited: 16/05/2013

ISSN (print): 1559-2294

ISSN (electronic): 1559-2308

Publisher: Landes Bioscience

URL: http://dx.doi.org/10.4161/epi.23416

DOI: 10.4161/epi.23416

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