Browse by author
Lookup NU author(s): Dr Cindy LeeORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
This study presents the application of the porous poly(D,L-lactic-co-glycolic acid) (PLGA) sponges fabricated from an organic solvent free supercritical gas foaming technique. Two formulations of PLGA sponges with different co-polymer compositions (85:15 and 50:50) were fabricated as novel scaffolds to guide human hepatoma cell line, Hep3B cell growth in vitro. The PLGA sponges showed desirable biodegradability and exhibited uniform pore size distribution with moderate interconnectivity. It was observed in this study that cells cultured on PLGA sponges showed lower proliferation rate as compared to the control during 14 days of culture as measured by using total DNA and methylthiazol tetrazolium (MTT) assays. However, the cells cultured on the sponges tended to aggregate to form cell islets which were able to express better hepatic functions. The enzyme-linked immunosorbent assay (ELISA) results showed that the cell-sponge constructs secreted 1.5–3.0 times more albumin than the control when normalized to cellular content. In a similar fashion, its detoxification ability was also predominantly higher than that of the control as indicated by the ethoxyresorufin-O-deethylase (EROD) results. By comparing the cells growing on the two formulations of PLGA sponges, it was found that the PLGA 85:15 sponge exhibited better conductive and desirable environment for hep3B cells as justified by better cell infiltration, higher proliferation and hepatic function than the PLGA 50:50 sponge. Biotechnol. Bioeng. 2008;100: 998–1009. 2008 Wiley Periodicals, Inc.
Author(s): Zhu XH, Lee LY, Hong JS, Tong YW, Wang CH
Publication type: Article
Publication status: Published
Journal: Biotechnology and Bioengineering
Year: 2008
Volume: 100
Issue: 5
Pages: 998-1009
ISSN (print): 0006-3592
ISSN (electronic): 1097-0290
Publisher: John Wiley & Sons, Inc.
URL: http://dx.doi.org/10.1002/bit.21824
DOI: 10.1002/bit.21824
Altmetrics provided by Altmetric