Toggle Main Menu Toggle Search

Open Access padlockePrints

Pseudonocardia hispaniensis sp. nov., a novel actinomycete isolated from industrial wastewater activated sludge

Lookup NU author(s): Professor Michael Goodfellow


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A novel actinomycete, designated PA3(T), was isolated from an oil refinery wastewater treatment plant, located in Palos de la Frontera, Huelva, Spain, and characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct subclade in the Pseudonocardia tree together with Pseudonocardia asaccharolytica DSM 44247(T). The chemotaxonomic properties of the isolate, for example, the presence of MK-8 (H-4) as the predominant menaquinone and iso-C-16:0 as the major fatty acid, are consistent with its classification in the genus Pseudonocardia. DNA:DNA pairing experiments between the isolate and the type strain of P. asaccharolytica DSM 44247(T) showed that they belonged to separate genomic species. The two strains were readily distinguished using a combination of phenotypic properties. Consequently, it is proposed that isolate PA3(T) represents a novel species for which the name Pseudonocardia hispaniensis sp. nov. is proposed. The type strain is PA3(T) (= CCM 8391(T) = CECT 8030(T)).

Publication metadata

Author(s): Cuesta G, Soler A, Alonso JL, Ruvira MA, Lucena T, Arahal DR, Goodfellow M

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek: International Journal of General and Molecular Microbiology

Year: 2013

Volume: 103

Issue: 1

Pages: 135-142

Print publication date: 17/08/2012

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer


DOI: 10.1007/s10482-012-9792-1


Altmetrics provided by Altmetric