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Lookup NU author(s): Mohamed Elbadawey, Robert Shields, Professor Nicholas JakubovicsORCiD, Professor Grant Burgess
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Objective. The growth of biofilms on tracheoesophageal speech valves shortens their life span and produces a reservoir of pathogens that may infect the respiratory tract. The authors have discovered a novel nontoxic deoxyribonuclease, NucB, from a marine isolate of Bacillus licheniformis that is effective at dispersing a variety of mono and mixed-species bacterial biofilms. The aim of this preliminary study was to determine whether NucB could also disrupt and remove mixed-species biofilms from tracheoesophageal speech valves. Study Design. Laboratory-based treatment and analysis of discarded tracheoesophageal speech valves. Setting. University human biology laboratory and the Department of Speech and Language Therapy at a tertiary referral hospital. Subjects and Methods. Seventeen ex vivo tracheoesophageal speech valves fouled with natural human biofilms were collected and divided into 2 equal parts. One half was treated with NucB and the other half with a control buffer solution. Biofilm removal was measured by microscopy and by culture of dispersed biofilm organisms on agar plates. Results. Significantly more organisms were released from biofilms using NucB than with buffer solution alone. On nonselective medium, more organisms were cultured in 11 samples (65%, n = 17, P < .05). Using growth media favoring fungi, more organisms were cultured in 14 samples (82%, n = 17, P < .05). Conclusion. The nontoxic deoxyribonuclease NucB was effective in releasing more microorganisms from biofilms on tracheoesophageal speech valves. This reflects its potential ability to break up and disperse these biofilms. Future studies will aim to develop NucB as a novel agent to prolong the life span of tracheoesophageal speech valves, thus reducing health care costs.
Author(s): Shakir A, ElBadawey MR, Shields RC, Jakubovics NS, Burgess JG
Publication type: Article
Publication status: Published
Journal: Otolaryngology: Head and Neck Surgery
Year: 2012
Volume: 147
Issue: 3
Pages: 509-514
Print publication date: 02/04/2012
ISSN (print): 0194-5998
ISSN (electronic): 1097-6817
Publisher: Sage Publications, Inc.
URL: http://dx.doi.org/10.1177/0194599812442867
DOI: 10.1177/0194599812442867
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