Toggle Main Menu Toggle Search

Open Access padlockePrints

Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation

Lookup NU author(s): Professor Bert van den Berg

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Bacterial biodegradation of hydrocarbons, an important process for environmental remediation, requires the passage of hydrophobic substrates across the cell membrane. Here, we report crystal structures of two outer membrane proteins, Pseudomonas putida TodX and Ralstonia pickettii TbuX, which have been implicated in hydrocarbon transport and are part of a subfamily of the FadL fatty acid transporter family. The structures of TodX and TbuX show significant differences with those previously determined for Escherichia coli FadL, which may provide an explanation for the substrate-specific transport of TodX and TbuX observed with in vivo transport assays. The TodX and TbuX structures revealed 14-stranded β-barrels with an N-terminal hatch domain blocking the barrel interior. A hydrophobic channel with bound detergent molecules extends from the extracellular surface and is contiguous with a passageway through the hatch domain, lined by both hydrophobic and polar or charged residues. The TodX and TbuX structures support a mechanism for transport of hydrophobic substrates from the extracellular environment to the periplasm via a channel through the hatch domain.


Publication metadata

Author(s): Hearn EM, Patel DR, van den Berg B

Publication type: Article

Publication status: Published

Journal: Proceedings of the National Academy of Sciences

Year: 2008

Volume: 105

Issue: 25

Pages: 8601-8606

ISSN (print): 0027-8424

ISSN (electronic): 1091-6490

Publisher: National Academy of Sciences

URL: http://dx.doi.org/10.1073/pnas.0801264105

DOI: 10.1073/pnas.0801264105


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share