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Crystal structure of the bacterial nucleoside transporter Tsx

Lookup NU author(s): Professor Bert van den Berg

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Abstract

Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded -barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.

Publication metadata

Author(s): Ye J, van den Berg B

Publication type: Article

Publication status: Published

Journal: EMBO Journal

Year: 2004

Volume: 23

Issue: 16

Pages: 3187-3195

ISSN (print): 0261-4189

ISSN (electronic): 1460-2075

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/sj.emboj.7600330

DOI: 10.1038/sj.emboj.7600330

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