Toggle Main Menu Toggle Search

Open Access padlockePrints

Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis

Lookup NU author(s): Graham Scholefield, Professor Heath MurrayORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

When starved, Bacillus subtilis cells can enter the developmental programme of endospore formation by activation of the master transcriptional regulator Spo0A. Correct chromosome copy number is crucial for the production of mature and fully resistant spores. The production and maintenance of one chromosome for the mother cell and one copy for the forespore requires accurate co-ordination between DNA replication and initiation of sporulation. Here, we show that Spo0A regulates chromosome copy number by directly binding to a number of Spo0A binding sites that are present near the origin of replication (oriC). We demonstrate that cells lacking three specific Spo0A binding sites at oriC display increased chromosome copy numbers when sporulation is induced. Our data support the hypothesis that Spo0A directly controls DNA replication during sporulation by binding to oriC.


Publication metadata

Author(s): Scholefield G; Murray H; Boonstra M; de Jong IG; Kuipers OP; Veening JW

Publication type: Article

Publication status: Published

Journal: Molecular Microbiology

Year: 2013

Volume: 87

Issue: 4

Pages: 925-938

Print publication date: 21/01/2013

ISSN (print): 0950-382X

ISSN (electronic): 1365-2958

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1111/mmi.12141

DOI: 10.1111/mmi.12141


Altmetrics

Altmetrics provided by Altmetric


Share