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The tumorigenicity-promoting activity of C-FABP in prostate cancer cells depends on its fatty acid-binding ability

Lookup NU author(s): Dr Imad Malki


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BackgroundCutaneous fatty acid binding protein(C-FABP) was identified by our research group as a gene involved in malignant progression of prostate cancer and able to promote the growth of primary tumours and induce metastasis when transfected into rat benign Rama 37 model cellsMethodTo study the possible relationship between the tumourigencity-promoting activity of cutaneous fatty acid binding protein (C-FABP) and its fatty acid-binding capability, mutations were generated on the fatty acid-binding motif of the C-FABP cDNA. The wild type and mutated C-FABP cDNAs cloned into E.Coli, to generate a clone expressing a wild type C-FABP which can bind to fatty acids; and two clones expressing mutated C-FABPs which are less capable of binding to fatty acidsResultsThe increased wild type C-FABP expression in prostate cells significantly increased cell proliferation, invasiveness, and tumourigencity in-vitro, whereas, the increased expression of both mutant forms of C-FABP did not significantly change these characteristics. When inoculated in nude mouse, 7 out of 8 mice produced tumours in the wild type group. In single mutant group, 4 out of 8 mice produced tumours. Whereas in the double mutant and control group, only 3 out of 8 mice produced tumours, respectivelyConclusionOur results suggest that the biological ability of C-FABP to promote tumourigencity of the prostate cancer cells depends on its ability of binding to fatty acids. Thus C-FABP may facilitate cancer development through transporting fatty acids into cells

Publication metadata

Author(s): Malki MI, Bao ZZ, Forootan S, Foster CS, Ke Y

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: 8th NCRI Cancer Conference

Year of Conference: 2012

Publisher: NCRI