ePrints

A facile in situ morphological characterization of smart genipin-crosslinked chitosan–poly(vinyl pyrrolidone) hydrogels

Lookup NU author(s): Glenn Hurst, Professor Katarina NovakovicORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Stable, responsive and autofluorescent genipin-crosslinked chitosan–poly(vinyl pyrrolidone) hydrogels have been synthesized. Morphological characterization techniques such as scanning electron microscopy, environmental scanning electron microscopy, and in situ confocal laser scanning microscopy (CLSM), in both reflectance and fluorescence modes, have been compared for their suitability to characterize the network structure of these hydrogels. CLSM is shown to be the optimal technique owing to the facile generation of the three-dimensional porous architecture and extra topographical information while the sample is immersed in the aqueous solution to which it will find application. CLSM is used in both reflectance and fluorescence modes to follow morphology variation as a function of time during swelling. Conveniently, acquisition via reflectance produces images with a higher degree of structural detail than fluorescence, widening the application of this method to characterize hydrogels where addition of a fluorescent probe, which may alter the native structure, is undesired.

Publication metadata

Author(s): Hurst GA, Novakovic K

Publication type: Article

Publication status: Published

Journal: Journal of Materials Research

Year: 2013

Pages: 1-8

Print publication date: 23/05/2013

ISSN (print): 0884-2914

ISSN (electronic): 2044-5326

Publisher: Cambridge University Press

URL: http://dx.doi.org/10.1557/jmr.2013.134

DOI: 10.1557/jmr.2013.134

Altmetrics

Funding

Share