ePrints

Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy

Lookup NU author(s): Professor Hermann Josef Vormoor

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

The development of stem-cell gene therapy is hindered by the absence of repopulation assays for primitive human hematopoietic cells. Current methods of gene transfer rely on in vitro colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, as well as inference from other mammalian species. We have identified a novel human hematopoietic cell, the SCID-repopulating cell (SRC), a cell more primitive than most LTC-ICs and CFCs. The SRC, exclusively present in the CD4+CD8- fraction, is capable of multilineage repopulation of the bone marrow of nonobese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice). SRCs were rarely transduced with retroviruses, distinguishing them from most CFCs and LTC-ICs. This observation is consistent with the low level of gene marking seen in human gene therapy trials. An SRC assay may aid in the characterization of hematopoiesis, as well as the improvement of transduction methods.

Publication metadata

Author(s): Larochelle A, Vormoor J, Hanenberg H, Wang JCY, Bhatia M, Lapidot T, Moritz T, Murdoch B, Xiao XL, Kato I, Williams DA, Dick JE

Publication type: Article

Publication status: Published

Journal: Nature Medicine

Year: 1996

Volume: 2

Issue: 12

Pages: 1329-1337

Print publication date: 01/12/1996

ISSN (print): 1078-8956

ISSN (electronic): 1546-170X

URL: http://dx.doi.org/10.1038/nm1296-1329

DOI: 10.1038/nm1296-1329

Notes: 1078-8956 (Print) Journal Article

Altmetrics

Share