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Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Lookup NU author(s): Dr Gabriel Uguru, Madhav Mondhe, Dr James StachORCiD



We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.

Publication metadata

Author(s): Uguru GC, Mondhe M, Goh S, Hesketh A, Bibb MJ, Good L, Stach JEM

Publication type: Article

Publication status: Published

Journal: PLoS ONE

Year: 2013

Volume: 8

Issue: 6

Print publication date: 27/06/2013

Date deposited: 29/11/2013

ISSN (electronic): 1932-6203

Publisher: Public Library of Science


DOI: 10.1371/journal.pone.0067509


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Funder referenceFunder name
BB/E017053/1Biotechnology and Biological Sciences Research Council (BBSRC)