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Lookup NU author(s): Dr Catherine Tétard-Jones, Professor Angharad MR GatehouseORCiD, Dr Julia Cooper, Professor Carlo Leifert, Professor Stephen Rushton
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
‘Omics analysis (transcriptomics, proteomics) quantifies changes in gene/protein expression, providing a snapshot of changes in biochemical pathways over time. Although tools such as modelling that are needed to investigate the relationships between genes/proteins already exist, they are rarely utilised. We consider the potential for using Structural Equation Modelling to investigate protein-protein interactions in a proposed Rubisco protein degradation pathway using previously published data from 2D electrophoresis and mass spectrometry proteome analysis. These informed the development of a prior model that hypothesised a pathway of Rubisco Large Subunit and Small Subunit degradation, producing both primary and secondary degradation products. Whilst some of the putative pathways were confirmed by the modelling approach, the model also demonstrated features that had not been originally hypothesised. We used Bayesian analysis based on Markov Chain Monte Carlo simulation to generate output statistics suggesting that the model had replicated the variation in the observed data due to protein-protein interactions. This study represents an early step in the development of approaches that seek to enable the full utilisation of information regarding the dynamics of biochemical pathways contained within proteomics data. As these approaches gain attention, they will guide the design and conduct of experiments that enable ‘Omics modelling to become a common place practice within molecular biology.
Author(s): Tétard-Jones C, Gatehouse AMR, Cooper J, Leifert C, Rushton S
Publication type: Article
Publication status: Published
Journal: PLoS ONE
Year: 2014
Volume: 9
Issue: 2
Print publication date: 03/02/2014
Online publication date: 03/02/2014
Acceptance date: 23/12/2013
Date deposited: 06/02/2014
ISSN (electronic): 1932-6203
Publisher: Public Library of Science
URL: http://dx.doi.org/10.1371/journal.pone.0087597
DOI: 10.1371/journal.pone.0087597
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