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Anaerobic degradation of 4-methylbenzoate via a specific 4-methylbenzoyl-CoA pathway

Lookup NU author(s): Dr Sven LahmeORCiD

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Abstract

The pathway for anaerobic degradation of 4-methylbenzoate was studied in the denitrifying alphaproteobacterium Magnetospirillum sp. strain pMbN1. Adaptation studies with whole cells indicated substrate-dependent induction of the capacity to degrade 4-methylbenzoate. Differential protein profiling (2D-DIGE) of 4-methylbenzoate- in comparison with benzoate- or succinate-adapted cells revealed the specific abundance increase of substrate-specific protein sets. Their coding genes form distinct clusters on the genome, two of which were assigned to 4-methylbenzoate and one to benzoate degradation. The predicted functions of the gene products agree with a specific 4-methylbenzoyl-CoA degradation pathway in addition to and analogous to the known anaerobic benzoyl-CoA degradation pathway. In vitro benzoyl-CoA and 4-methylbenzoyl-CoA reductase activities revealed the electron donor and ATP-dependent formation of the corresponding conjugated cyclic dienoyl-CoA/4-methyl-dienoyl-CoA products. The 4-methylbenzoyl-CoA reductase activity was induced in the presence of 4-methylbenzoate. In accordance, metabolite analysis of cultures grown with 4-methylbenzoate tentatively identified 4-methylcyclohex-1,5-diene-1-carboxylate. The 4-methylbenzoate induced genes were assigned to code for the putative 4-methylbenzoyl-CoA reductase; their products display pronounced sequence disparity from the conventional class I benzoyl-CoA reductase, which does not accept substituents at the para-position. Identification of 3-methylglutarate together with the formation of specific proteins for ring cleavage and β-oxidation in 4-methylbenzoate-adapted cells suggest conservation of the methyl group along the specific 4-methylbenzoyl-CoA degradation pathway.

Publication metadata

Author(s): Lahme S, Eberlein C, Jarling R, Kube M, Boll M, Wilkes H, Reinhardt R, Rabus R

Publication type: Article

Publication status: Published

Journal: Environmental Microbiology

Year: 2012

Volume: 14

Issue: 5

Pages: 1118-1132

Print publication date: 20/01/2012

ISSN (print): 1462-2912

ISSN (electronic): 1462-2920

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1111/j.1462-2920.2011.02693.x

DOI: 10.1111/j.1462-2920.2011.02693.x

PubMed id: 22264224

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