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Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis

Lookup NU author(s): Dr Natalie Ferry, Dr Martin EdwardsORCiD, Professor Angharad MR GatehouseORCiD, Professor Kanglai He

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Abstract

The full-length cDNA of four Ofapn aminopeptidases were cloned and sequenced from susceptible and resistant Ostrinia furnacalis strains. Four sequences were identified as APN because they shared the common structural features with APN from Lepidoptera, including the signal peptide, GPI anchor signal, the zinc binding/gluzincin motif HEX2HX18E and the gluzincin aminopeptidase motif GAMEN. Compared with APN sequences from the susceptible strain, there were 9, 5, 10 and 12 amino acid variations in the deduced protein sequences from the resistant strain. There were also differences in mRNA expression of the four Ofapn genes between resistant and susceptible O. furnacalis strains. (C) 2013 Published by Elsevier Inc.

Publication metadata

Author(s): Xu LN, Wang ZY, Zhang J, Ferry N, Edwards MG, Gatehouse AMR, He KL

Publication type: Article

Publication status: Published

Journal: Journal of Invertebrate Pathology

Year: 2014

Volume: 115

Pages: 95-98

Print publication date: 21/11/2013

ISSN (print): 0022-2011

ISSN (electronic): 1096-0805

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.jip.2013.11.001

DOI: 10.1016/j.jip.2013.11.001

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