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Lookup NU author(s): Dr Steven LisgoORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
During brain development, neural stem cells (NSCs) receive on-or-off signals important for regulating their amplification and reaching adequate neuron density. However, how a coordinated regulation of intracellular pathways and genetic programs is achieved has remained elusive. Here, we found that the embryonic (e) CSF contains 10(12) nanoparticles/ml (77 nm diameter), some of which were identified as exosome nanovesicles that contain evolutionarily conserved molecules important for coordinating intracellular pathways. eCSF nanovesicles collected from rodent and human embryos encapsulate protein and microRNA components of the insulin-like growth factor (IGF) signaling pathway. Supplementation of eCSF nanovesicles to a mixed culture containing eNSCs activated the IGF-mammalian target of rapamycin complex 1 (mTORC1) pathway in eNSCs and expanded the pool of proliferative eNSCs. These data show that the eCSF serves as a medium for the distribution of nanovesicles, including exosomes, and the coordinated transfer of evolutionary conserved molecules that regulate eNSC amplification during corticogenesis.
Author(s): Feliciano DM, Zhang SL, Nasrallah CM, Lisgo SN, Bordey A
Publication type: Article
Publication status: Published
Journal: PLoS One
Print publication date: 12/02/2014
Acceptance date: 12/01/2014
Date deposited: 16/04/2014
ISSN (electronic): 1932-6203
Publisher: Public Library of Science
Notes: ARTN e88810
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