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Biodentine and Mineral Trioxide Aggregate Induce Similar Cellular Responses in a Fibroblast Cell Line

Lookup NU author(s): Dr Helen Bosomworth, Dr Claire Field, Professor John Whitworth, Professor Ruth Valentine


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Introduction: The aim of this study was to assess the cell viability and messenger RNA expression of interleukin (IL)-1 alpha and IL-6 in 3T3 fibroblast cells when in direct contact with Biodentine (Septodont, Saint Maur de Fosses, France) and mineral trioxide aggregate (MTA). Methods: Biodentine and MTA were coated onto coverslips and allowed to set. An uncoated coverslip and one coated with GC Fuji IX (GC Corporation, Tokyo, Japan) were used as controls. Coverslips were cultured with 3T3 fibroblast cells. Cell viability was assessed quantitatively using AlamarBlue dye (Serotec, Oxford, UK) after 3, 6, 24, and 72 hours. Morphologic cell changes of 3T3 cells in contact with BD and MTA were observed by scanning electron microscopy, and cytokine expression was assessed at the messenger RNA level by semiquantitative reverse-transcription polymerase chain reaction after 3 and 24 hours of direct contact with the materials. Results: Cells in contact with Biodentine and MTA showed similar viability to untreated control cells at all time points, with the exception of 6 hours when viability was decreased with both treatments. Examination by scanning electron microscopy revealed cells adhering to most of the Biodentine surface after 24 hours. However, for MTA samples, significantly fewer cells were observed. The messenger RNA expression of IL-1 alpha and IL-6 by cells in contact with Biodentine was similar to cells in contact with MTA. Conclusions: Biodentine and MTA showed similar cytotoxicity and induced a similar pattern of cytokine expression.

Publication metadata

Author(s): Nunez CMC, Bosomworth HJ, Field C, Whitworth JM, Valentine RA

Publication type: Article

Publication status: Published

Journal: Journal of Endodontics

Year: 2014

Volume: 40

Issue: 3

Pages: 406-411

Print publication date: 17/01/2014

ISSN (print): 0099-2399

ISSN (electronic): 1878-3554

Publisher: Elsevier Inc.


DOI: 10.1016/j.joen.2013.11.006


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