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Transcriptional response to vancomycin in a highly vancomycin-resistant Streptomyces coelicolor mutant

Lookup NU author(s): Dr Fernando Santos Beneit

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Abstract

Aim: The main objective of this study is to understand the mechanism of vancomycin resistance in a Streptomyces coelicolor disrupted mutant highly resistant to vancomycin. Materials & methods: Different techniques have been performed in the study including gene disruption, primer extension, antibiotic susceptibility tests, electron microscopy, confocal microscopy, cell wall analysis and microarrays.Results: During the phenotypical characterization of mutant strains affected in phosphate-regulated genes of unknown function, we found that the S. coelicolor SCO2594 disrupted mutant was highly resistant to vancomycin and had other phenotypic alterations such as antibiotic overproduction, impaired growth and reduction of phosphate cell wall content. Transcriptomic studies with this mutant indicated a relationship between vancomycin resistance and cell wall stress. Conclusion: We identified a S. coelicolor mutant highly resistant to vancomycin in both high and low phosphate media. In addition to Van proteins, others such as WhiB or SigE appear to be involved in this regulatory mechanism.

Publication metadata

Author(s): Santos-Beneit F, Fernández-Martínez LT, Rodríguez-García A, Martín-Martín S, Ordóñez-Robles M, Yagüe P, Manteca A, Martín JF

Publication type: Article

Publication status: Published

Journal: Future Microbiology

Year: 2014

Volume: 9

Pages: 603-622

Print publication date: 01/05/2014

ISSN (print): 1746-0913

ISSN (electronic): 1746-0921

Publisher: Future Medicine Ltd.

URL: http://dx.doi.org/10.2217/fmb.14.21

DOI: 10.2217/fmb.14.21

PubMed id: 24957088

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