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Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces

Lookup NU author(s): Dr John Perry, Professor Kate Gould


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Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin-cycloserine-egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile. A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C. difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C. difficile was 87.6% compared with 26.6% for CCEY/L (P < 0.0001). chromID C. difficile performed significantly better than CCEY/L for the recovery of C. difficile from the environment. (C) 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Hill KA, Collins J, Wilson L, Perry JD, Gould FK

Publication type: Article

Publication status: Published

Journal: Journal of Hospital Infection

Year: 2013

Volume: 83

Issue: 2

Pages: 164-166

Print publication date: 01/02/2013

Online publication date: 29/11/2012

Acceptance date: 07/10/2012

ISSN (print): 0195-6701

ISSN (electronic): 1532-2939

Publisher: W.B. Saunders Co. Ltd


DOI: 10.1016/j.jhin.2012.10.006


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