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BRCA1 mediates resistance to apoptosis in response to DNA-damaging agents, causing BRCA1 wild-type tumours to be significantly more resistant to DNA damage than their mutant counterparts. In this study, we demonstrate that following treatment with the DNA-damaging agents, etoposide or camptothecin, BRCA1 is required for the activation of nuclear factor-kappa B (NF-kappa B), and that BRCA1 and NF-kappa B cooperate to regulate the expression of the NF-kappa B antiapoptotic targets BCL2 and XIAP. We show that BRCA1 and the NF-kappa B subunit p65/RelA associate constitutively, whereas the p50 NF-kappa B subunit associates with BRCA1 only upon DNA damage treatment. Consistent with this BRCA1 and p65 are present constitutively on the promoters of BCL2 and XIAP, whereas p50 is recruited to these promoters only in damage treated cells. Importantly, we demonstrate that the recruitment of p50 onto the promoters of BCL2 and XIAP is dependent upon BRCA1, but independent of its NF-kappa B partner subunit p65. The functional relevance of NF-kappa B activation by BRCA1 in response to etoposide and camptothecin is demonstrated by the significantly reduced survival of BRCA1 wild-type cells upon NF-kappa B inhibition. This study identifies a novel BRCA1-p50 complex, and demonstrates for the first time that NF-kappa B is required for BRCA1-mediated resistance to DNA damage. It reveals a functional interdependence between BRCA1 and NF-kappa B, further elucidating the role played by NF-kappa B in mediating cellular resistance of BRCA1 wild-type tumours to DNA-damaging agents.
Author(s): Harte MT, Gorski JJ, Savage KI, Purcell JW, Barros EW, Burn PM, McFarlane C, Mullan PB, Kennedy RD, Perkins ND, Harkin DP
Publication type: Article
Publication status: Published
Print publication date: 01/01/2014
Online publication date: 25/02/2013
Acceptance date: 14/12/2012
ISSN (print): 0950-9232
ISSN (electronic): 1476-5594
Publisher: Nature Publishing Group
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