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Generation and epitope mapping of a sub-group cross-reactive anti-respiratory syncytial virus G glycoprotein monoclonal antibody which is protective in vivo

Lookup NU author(s): Dr Cheng Siang Tan, Fiona Fenwick, Dr Edward Routledge, Emeritus Professor Geoffrey Toms

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Abstract

Passively administered antibodies to conserved epitopes on the attachment (G) glycoprotein of human respiratory syncytial virus (hRSV) have potential in the immunoprophylaxis of human infections. This study set out to generate monoclonal antibodies (MAbs) recognizing all prevalent lineages of HRSV and capable of immunoprophylaxis in mice. Two murine MAbs of broad specificity for prevalent virus strains were generated by immunization of mice with hRSV of sub-group A followed by selection of hybridomas on recombinant G glycoprotein from a sub-group B virus. The anti-G hybridomas generated secreted antibody of high affinity but negligible neutralizing capacity one of which was tested in mice and found to be protective against live virus challenge. Western blotting and partial epitope mapping on transiently expressed G-glycoprotein fragments indicate that these antibodies recognize a complex epitope on the protein backbone of the molecule involving residues both C '- and N-terminal to the central conserved motif. J. Med. Virol. 86:1267-1277, 2014. (c) 2014 Wiley Periodicals, Inc.

Publication metadata

Author(s): Robinson MJ, Tan CS, Fenwick F, Chambers CJ, Routledge EG, Toms GL

Publication type: Article

Publication status: Published

Journal: Journal of Medical Virology

Year: 2014

Volume: 86

Issue: 7

Pages: 1267-1277

Print publication date: 01/07/2014

Online publication date: 10/01/2014

Acceptance date: 17/12/2013

ISSN (print): 0146-6615

ISSN (electronic): 1096-9071

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1002/jmv.23881

DOI: 10.1002/jmv.23881

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