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Directed differentiation of umbilical cord blood stem cells into cortical GABAergic neurons

Lookup NU author(s): Dr Nadhim Bayatti, Emerita Professor Susan Lindsay


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Umbilical cord blood contains a population of non-hematopoietic multipotent stem cells that are capable of neuronal differentiation in-vitro. These cells have shown great potential as a therapeutic tool for central nervous system diseases and disorders. However whether these cells are able to produce neurons with similar developmental and functional characteristics to indigenous neurons within the brain remains poorly investigated. In this study, we used purified umbilical cord blood non-hematopoietic stem cells to produced GABAergic neurons with similar developmental and functional characteristics to cortical GABAergic neurons. We analyzed the expression of transcription factors MASH1, DLX1 and DLX2 throughout the 24 days of a sequential neuronal induction protocol and found that their expression patterns resembled those reported in the developing human cortex. The derived neurons also expressed components of GABAergic neurotransmission including GABA regulatory enzymes, GABA receptor subunits and GABA transporters. Thus we have demonstrated that umbilical cord blood stem cells are capable of producing cortical-like GABAergic neurons in vitro. This highlights the potential of umbilical cord blood stem cells as a therapeutic tool for neural injuries and disorders.

Publication metadata

Author(s): Ali H, Bayatti N, Lindsay S, Dashti AA, Al-Mulla F

Publication type: Article

Publication status: Published

Journal: Acta Neurobiologiae Experimentalis

Year: 2013

Volume: 73

Issue: 2

Pages: 250-259

Print publication date: 01/01/2013

Acceptance date: 07/05/2013

ISSN (print): 0065-1400

ISSN (electronic): 1689-0035

Publisher: Polish Academy of Sciences, M. Nencki Institute of Experimental Biology



Funder referenceFunder name
03-1302-2012Kuwait Foundation for the Advancement for Sciences (KFAS)
GM01/01Research Core Facility (RCF) at Health Sciences Center at Kuwait University