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Examination of Parameters Influencing [3H]MK-801 Binding in Postmortem Human Cortex

Lookup NU author(s): Dr Margaret Piggott, Emeritus Professor Elaine Perry, Dr Arjun Sahgal, Emeritus Professor Robert Perry


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[H-3]MK-801 binding was used as an index of the glutamate receptor N-methyl-D-aspartate-subtype channel to examine the influence of gender, age, mode of death (agonal status), interval between death and autopsy (postmortem delay), and time in storage at -70-degrees-C in well washed homogenate preparations from postmortem human frontal cortex. Basal binding and the modulatory effects of glutamate, glycine, spermidine, and zinc were examined with respect to these variables. Basal binding was sensitive to agonal status, being higher in sudden death cases. The effect of added glutamate and glycine was sensitive to age, with a trend toward lower binding with increasing age. The effect of added spermidine alone was sensitive to storage time at -70-degrees-C, the binding being higher with longer storage time. The effect of added zinc was also sensitive to postmortem delay, with zinc causing a greater reduction in binding with shorter postmortem delays. Thus, with the exception of gender, all variables examined influenced [H-3]MK-801 binding, highlighting the attention that should be given to these factors in postmortem studies in normal and diseased human subjects.

Publication metadata

Author(s): Piggott MA, Perry EK, Sahgal A, Perry RH

Publication type: Article

Publication status: Published

Journal: Journal of Neurochemistry

Year: 1992

Volume: 58

Issue: 3

Pages: 1001-1008

Print publication date: 01/03/1992

ISSN (print): 0022-3042

ISSN (electronic): 1471-4159

Publisher: Wiley-Blackwell


DOI: 10.1111/j.1471-4159.1992.tb09354.x


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