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CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

Lookup NU author(s): Professor Georg Lietz, Peter Curtis, Professor John Mathers, Dr Franck Tourniaire

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Abstract

Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on a-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., beta-carotene, alpha-carotene, and beta-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level. J. Nutr. 143: 448-456, 2013.

Publication metadata

Author(s): Borel P, Lietz G, Goncalves A, de Edelenyi FS, Lecompte S, Curtis P, Goumidi L, Caslake MJ, Miles EA, Packard C, Calder PC, Mathers JC, Minihane AM, Tourniaire F, Kesse-Guyot E, Galan P, Hercberg S, Breidenassel C, Gross MG, Moussa M, Meirhaeghe A, Reboul E

Publication type: Article

Publication status: Published

Journal: Journal of Nutrition

Year: 2013

Volume: 143

Issue: 4

Pages: 448-456

Print publication date: 01/04/2013

Online publication date: 20/02/2013

Acceptance date: 06/01/2013

ISSN (print): 0022-3166

ISSN (electronic): 1541-6100

Publisher: American Society for Nutrition

URL: http://dx.doi.org/10.3945/jn.112.172734

DOI: 10.3945/jn.112.172734

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