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Structural Changes in the Hydrophobic Hinge Region Adversely Affect the Activity and Fidelity of the I260Q Mutator DNA Polymerase beta

Lookup NU author(s): Dr Susan Firbank

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Abstract

The I260Q variant of DNA polymerase beta is an efficient mutator polymerase with fairly indiscriminate misincorporation activities opposite all template bases. Previous modeling studies have suggested that I260Q harbors structural variations in its hinge region. Here, we present the crystal structures of wild type and I260Q rat polymerase beta in the presence and absence of substrates. Both the I260Q apoenzyme structure and the closed ternary complex with double-stranded DNA and ddTTP show ordered water molecules in the hydrophobic hinge near Gln260, whereas this is not the case in the wild type polymerase. Compared to wild type polymerase beta ternary complexes, there are subtle movements around residues 260, 272, 295, and 296 in the mutant. The rearrangements in this region, coupled with side chain movements in the immediate neighborhood of the dNTP-binding pocket, namely, residues 258 and 272, provide an explanation for the altered activity and fidelity profiles observed in the I260Q mutator polymerase.

Publication metadata

Author(s): Gridley CL, Rangarajan S, Firbank S, Dalal S, Sweasy JB, Jaeger J

Publication type: Article

Publication status: Published

Journal: Biochemistry

Year: 2013

Volume: 52

Issue: 25

Pages: 4422-4432

Print publication date: 25/06/2013

Online publication date: 07/05/2013

Acceptance date: 23/04/2013

ISSN (print): 0006-2960

ISSN (electronic): 1520-4995

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/bi301368f

DOI: 10.1021/bi301368f

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