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Lookup NU author(s): Francesca Migheli, Professor John Mathers
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The aim of this work was to assess the impact on measurements of methylation of a panel of four cancer gene promoters of purifying tumor cells from colorectal tissue samples using the epithelial cell adhesion molecule (EpCAM)-immunomagnetic cell enrichment approach. We observed that, on average, methylation levels were higher in enriched cell fractions than in the whole tissue, but the difference was significant only for one out of four studied genes. In addition, there were strong correlations between methylation values for individual samples of whole tissue and the corresponding enriched cell fractions. Therefore, assays on whole tissue are likely to provide reliable estimates of tumor-specific methylation of cancer genes. However, tumor cell tissue separation using immunomagnetic beads could, in some cases, give a more accurate value of gene promoter methylation than the analysis of the whole cancer tissue, although relatively expensive and time-consuming. The efficacy and feasibility of the immunomagnetic cell sorting for methylation studies are discussed.
Author(s): Failli A, Legitimo A, Migheli F, Coppede F, Mathers JC, Spisni R, Miccoli P, Migliore L, Consolini R
Publication type: Article
Publication status: Published
Journal: International Journal of Molecular Sciences
Year: 2014
Volume: 15
Issue: 1
Pages: 44-57
Print publication date: 01/01/2014
Online publication date: 20/12/2013
Acceptance date: 13/12/2013
Date deposited: 08/08/2014
ISSN (print): 1661-6596
ISSN (electronic): 1422-0067
Publisher: MDPI AG
URL: http://dx.doi.org/10.3390/ijms15010044
DOI: 10.3390/ijms15010044
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