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Standardisation of the factor H autoantibody assay

Lookup NU author(s): Rachael Horbury, Dr Eva-Maria Hunze, Professor Kevin MarchbankORCiD


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The screening of all atypical haemolytic uraemic syndrome (aHUS) patients for factor H autoantibodies is best practice. However, there is no consensus assay for the reporting of factor H autoantibody titres. In this study, three European complement laboratories with expertise in the field of autoantibody testing address this by systematically evaluating several ELISA methods used for the detection of factor H autoantibodies.All methods tested adequately detect high titre samples. However, this study recommends the Paris method for the detection and reporting of factor H autoantibodies to be used when setting up a factor H autoantibody screen. The importance of individual sample background subtraction in these ELISA tests was established. The use of a relative or arbitrary unit index with a common positive and negative serum allowed for consistent comparison of findings from different test centres. Therefore, it is recommended that a standard arbitrary unit scale based on a titration curve from a common positive anti-serum be adopted to allow future establishment of the relative importance of particular titres of factor H autoantibodies in aHUS.Systematic assay for the presence of factor H autoantibodies in patients using the Paris method will provide the longitudinal analysis needed to fully establish the importance of factor H autoantibodies in disease. This will feed into additional research to clarify whether additional factors have a bearing on the phenotype/outcome of autoimmune aHUS. (C) 2013 Elsevier GmbH. All rights reserved.

Publication metadata

Author(s): Watson R, Lindner S, Bordereau P, Hunze EM, Tak F, Ngo S, Zipfel PF, Skerka C, Dragon-Durey MA, Marchbank KJ

Publication type: Article

Publication status: Published

Journal: Immunobiology

Year: 2014

Volume: 219

Issue: 1

Pages: 9-16

Print publication date: 01/01/2014

ISSN (print): 0171-2985

Publisher: Elsevier


DOI: 10.1016/j.imbio.2013.06.004


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Funder referenceFunder name
Boehrigner Ingelheim Foundation
Interdisciplinary Center for Clinical Research (IZKF) of the University Hospital in Jena
2012-305608European Community