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A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus

Lookup NU author(s): Dr Steve Boneham

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Abstract

Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis.

Publication metadata

Author(s): McClure P, Curran R, Boneham S, Ball JK

Publication type: Article

Publication status: Published

Journal: Journal of Virological Methods

Year: 2000

Volume: 88

Issue: 1

Pages: 73-80

Print publication date: 26/07/2000

Online publication date: 27/07/2000

Acceptance date: 06/04/2000

ISSN (print): 0166-0934

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0166-0934(00)00179-8

DOI: 10.1016/S0166-0934(00)00179-8

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