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A molecular diagnostic approach able to detect the recurrent genetic prognostic factors typical of presenting myeloma

Lookup NU author(s): Professor Graham Jackson



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Risk stratification in myeloma requires an accurate assessment of the presence of a range of molecular abnormalities including the differing IGH translocations and the recurrent copy number abnormalities that can impact clinical behavior. Currently, interphase fluorescence in situ hybridization is used to detect these abnormalities. High failure rates, slow turnaround, cost, and labor intensiveness make it difficult and expensive to use in routine clinical practice. Multiplex ligation-dependent probe amplification (MLPA), a molecular approach based on a multiplex polymerase chain reaction method, offers an alternative for the assessment of copy number changes present in the myeloma genome. Here, we provide evidence showing that MLPA is a powerful tool for the efficient detection of copy number abnormalities and when combined with expression assays, MLPA can detect all of the prognostically relevant molecular events which characterize presenting myeloma. This approach opens the way for a molecular diagnostic strategy that is efficient, high throughput, and cost effective. (c) 2014 The Authors. Genes, Chromosomes & Cancer Published by Wiley Periodicals, Inc.

Publication metadata

Author(s): Boyle EM, Proszek PZ, Kaiser MF, Begum D, Dahir N, Savola S, Wardell CP, Leleu X, Ross FM, Chiecchio L, Cook G, Drayson MT, Owen RG, Ashcroft JM, Jackson GH, Child JA, Davies FE, Walker BA, Morgan GJ

Publication type: Article

Publication status: Published

Journal: Genes, Chromosomes and Cancer

Year: 2015

Volume: 54

Issue: 2

Pages: 91-98

Print publication date: 01/02/2015

Online publication date: 07/10/2014

Acceptance date: 11/09/2014

Date deposited: 07/08/2015

ISSN (print): 1045-2257

ISSN (electronic): 1098-2264

Publisher: Wiley-Blackwell


DOI: 10.1002/gcc.22222


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Funder referenceFunder name
Deutsche Forschungsgemeinschaft
Myeloma UK Programme Grant
Schering Health Care
UK Medical Research Council
University of Leeds
Cancer Research UK Sample Collection Grant (CTAAC)
Federation Francaise de Recherche sur le myelome et les gammapathies
National Institutes of Health Biomedical Research Centre at the Royal Marsden Hospital
Ortho Biotech
KA 3338/1-1