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The subgingival microbiome of clinically healthy current and never smokers

Lookup NU author(s): Professor Philip Preshaw

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Abstract

Dysbiotic oral bacterial communities have a critical role in the etiology and progression of periodontal diseases. The goal of this study was to investigate the extent to which smoking increases risk for disease by influencing the composition of the subgingival microbiome in states of clinical health. Subgingival plaque samples were collected from 200 systemically and periodontally healthy smokers and nonsmokers. 16S pyrotag sequencing was preformed generating 1 623 713 classifiable sequences, which were compared with a curated version of the Greengenes database using the quantitative insights into microbial ecology pipeline. The subgingival microbial profiles of smokers and never-smokers were different at all taxonomic levels, and principal coordinate analysis revealed distinct clustering of the microbial communities based on smoking status. Smokers demonstrated a highly diverse, pathogen-rich, commensal-poor, anaerobic microbiome that is more closely aligned with a disease-associated community in clinically healthy individuals, suggesting that it creates an at-risk-for-harm environment that is primed for a future ecological catastrophe.

Publication metadata

Author(s): Mason MR, Preshaw PM, Nagaraja HN, Dabdoub SM, Rahman A, Kumar PS

Publication type: Article

Publication status: Published

Journal: ISME Journal

Year: 2015

Volume: 9

Issue: 1

Pages: 268-272

Online publication date: 11/07/2014

Acceptance date: 02/06/2014

ISSN (print): 1751-7362

ISSN (electronic): 1751-7370

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/ismej.2014.114

DOI: 10.1038/ismej.2014.114

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