Browse by author
Lookup NU author(s): Professor Hans-Peter Klenk
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258T) to 97.8 % (Actinokineospora baliensis NBRC 104211T). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H4). The major fatty acids were found to be iso-C16:0, iso-C15:0, iso-C16:1 H and iso-C16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84T (=DSM 45961T = CECT 8578T).
Author(s): Aouiche A, Bouras N, Mokrane S, Zitouni A, Schumann P, Spröer C, Sabaou N, Klenk HP
Publication type: Article
Publication status: Published
Journal: Antonie van Leeuwenhoek
Print publication date: 01/01/2015
Online publication date: 07/11/2014
Acceptance date: 01/11/2014
ISSN (print): 0003-6072
ISSN (electronic): 1572-9699
Altmetrics provided by Altmetric