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Lookup NU author(s): Professor Hans-Peter Klenk
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A novel halophilic, filamentous actinomycete, designated H254T, was isolated from a Saharan soil sample collected from Biskra (Northern Sahara), and subjected to a polyphasic taxonomic characterization. The strain is Gram-positive, aerobic, and halophilic, and the optimum NaCl concentration for growth is 15–20 % (w/v). The cell-wall hydrolysate contained meso-diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H4) was the predominant menaquinone. The major fatty acid profiles were anteiso-C17:0 (32.8 %), C15:0 (28 %), and iso-C17:0 (12.3 %). Comparative analysis of the 16S rRNA gene sequences revealed that the strain H254T formed a well-separated sub-branch within the radiation of the genus Actinopolyspora, and the microorganism was most closely related to Actinopolyspora saharensis DSM 45459T (99.2 %), Actinopolyspora halophila DSM 43834T (99.1 %), and Actinopolyspora algeriensis DSM 45476T (99.0 %). Nevertheless, the strain had relatively lower mean values for DNA–DNA relatedness with the above strains (57.2, 68.4, and 45.6 %, respectively). Based on phenotypic features and phylogenetic position, we propose that strain H254T represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora biskrensis sp. nov. is proposed. The type strain of A. biskrensis is strain H254T (=DSM 46684T =CECT 8576T).
Author(s): Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Spröer C, Klenk HP, Sabaou N
Publication type: Article
Publication status: Published
Journal: Current Microbiology
Print publication date: 01/03/2015
Online publication date: 28/11/2014
Acceptance date: 29/10/2014
ISSN (print): 0343-8651
ISSN (electronic): 1432-0991
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