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Streptomyces zagrosensis sp. nov., isolated from soil

Lookup NU author(s): Dr Maria Del Carmen Montero-CalasanzORCiD, Professor Hans-Peter Klenk

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Abstract

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces. On ISP2 medium, strain HM 1154^T produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained ll-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H₄) and MK-9(H₂) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C_{16 : 0} and anteiso-C_{15 : 0}. Strain HM 1154^T exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146^T (99.4 %), Streptomyces varsoviensis DSM 40346^T (99.3 %), Streptomyces youssoufiensis DSM 41920^T (99.2 %), Streptomyces abikoensis DSM 40831^T (99.2 %), Streptomyces rimosus subsp. rimosus DSM 40260^T (99.1 %), Streptomyces luteireticuli DSM 40509^T (99.1 %), Streptomyces thioluteus DSM 40027^T (99.1 %), Streptomyces blastmyceticus DSM 40029^T (99.0 %) and Streptomyces hiroshimensis DSM 40037^T (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154^T is considered to represent a novel species within the genus Streptomyces, for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154^T ( = DSM 42018^T = UTMC 1154^T = CECT 8305^T).

Publication metadata

Author(s): Mohammadipanah F, Hamedi J, Sproer C, Rohde M, Montero-Calasanz MDC, Klenk HP

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2014

Volume: 64

Issue: 10

Pages: 3434-3440

Print publication date: 01/10/2014

Online publication date: 16/07/2014

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Society for General Microbiology

URL: http://dx.doi.org/10.1099/ijs.0.064527-0

DOI: 10.1099/ijs.0.064527-0

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